To estimate the Net Primary Production (NPP) of an aquatic ecosystem
Aim :- To estimate the Net Primary Production (NPP) of an aquatic ecosystem. Apparatus :- - Light and dark bottles (BOD bottles or similar) - Water sampler (e.g., Van Dorn sampler) - turbidity meter - pH meter - Temperature probe - Dissolved oxygen (DO) meter or oxygen electrodes - Stopclock or timer - Calculator or computer with statistical software Theory :- - Net Primary Production (NPP) is the rate at which plants produce organic matter through photosynthesis - The light and dark bottle method measures the change in dissolved oxygen (DO) levels in the water over time - The difference in DO levels between the light and dark bottles represents the NPP Chemicals Used :- 1. *Sodium thiosulfate (Na2S2O3)*: Used to remove chlorine and chloramines from the water sample, which can interfere with DO measurements. 2. *Manganese sulfate (MnSO4)*: Used as a catalyst to facilitate the oxidation of iodide to iodine in the Winkler method for DO meas...